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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNNI3 / Cardiac Troponin I Antibody IHC‑plus™ LS‑B2268
Note: This antibody replaces LS-C39879
Cardiac Troponin I antibody LS-B2268 is an unconjugated mouse monoclonal antibody to Cardiac Troponin I (TNNI3) from human, mouse, bovine and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2268-50
50 µg (1 mg/ml)
$515
Description
Cardiac Troponin I antibody LS-B2268 is an unconjugated mouse monoclonal antibody to Cardiac Troponin I (TNNI3) from human, mouse, bovine and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Bovine TNNI3 / Cardiac Troponin I
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rabbit, Chicken, Frog (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 5 µg/ml)
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Native bovine cardiac Troponin I.
Specificity
Recognizes bovine Troponin I
Usage
Immunohistochemistry: LS-B2268 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2268 was determined to be 2.5-5 ug/ml.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
About TNNI3 / Cardiac Troponin I
P19429 NM_000363 NP_000354.4

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Immunohistochemistry

Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Requested From: United States
Date Requested: 12/11/2018
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