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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNNI3 / Cardiac Troponin I Antibody (C-Terminus) IHC-plus™ LS-B5726

Note: This antibody replaces LS-C80415
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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TNNI3 / Cardiac Troponin I Antibody (C-Terminus) IHC-plus™ LS-B5726

Note: This antibody replaces LS-C80415
Antibody:
Rabbit Polyclonal to Human TNNI3 / Cardiac Troponin I
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5726-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TNNI3 / Cardiac Troponin I
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Cardiac Troponin I antibody LS-B5726 is an unconjugated rabbit polyclonal antibody to Cardiac Troponin I (TNNI3) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human TNNI3 / Cardiac Troponin I
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from C-Terminus of human TNNI3 (Q59H18, NP_000354). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Cat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Catfish, Zebrafish (92%); Platypus, Seabass (85%); Gorilla, Baboon, Elephant, Bat, Turkey, Zebra finch, Chicken, Armadillo, Lizard, Sablefish (84%); Xenopus (83%).
Blocking Peptide
LS-E15226 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5726. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human TNNI3 / CTnI
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNNI3 / Cardiac Troponin I
P19429 NM_000363 NP_000354.4

Validation

Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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