Cardiac Troponin I antibody LS-B5726 is an unconjugated rabbit polyclonal antibody to Cardiac Troponin I (TNNI3) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human TNNI3 / Cardiac Troponin I
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
Synthetic peptide from C-Terminus of human TNNI3 (Q59H18, NP_000354). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Cat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Catfish, Zebrafish (92%); Platypus, Seabass (85%); Gorilla, Baboon, Elephant, Bat, Turkey, Zebra finch, Chicken, Armadillo, Lizard, Sablefish (84%); Xenopus (83%).
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
About TNNI3 / Cardiac Troponin I