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TNNI3 / Cardiac Troponin I Antibody (aa67-78) LS-C186616

TNNI3 antibody (1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TNNI3 / Cardiac Troponin I Antibody (aa67-78) LS-C186616

Antibody:
Goat Polyclonal to Human TNNI3 / Cardiac Troponin I
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Goat, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405 
LS-C186616-100
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human TNNI3 / Cardiac Troponin I
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Goat, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Cardiac Troponin I antibody LS-C186616 is an unconjugated goat polyclonal antibody to Cardiac Troponin I (TNNI3) from human, bovine, dog and other species. Validated for Peptide-ELISA and WB.

Target
Human TNNI3 / Cardiac Troponin I
Host
Goat
Reactivity
Human, Monkey, Bovine, Dog, Goat, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
Peptide with sequence C-ERRGEKGRALST, from the internal region of the protein sequence according to NP_000354.4.
Blocking Peptide
LS-E28046 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186616. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa67-78
Specificity
Human TNNI3 / CTnI
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 27kD band observed in Human Heart lysates (calculated MW of 24.0kD according to NP_000354.4). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNNI3 / Cardiac Troponin I
P19429 NM_000363 NP_000354.4

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Requested From: United States
Date Requested: 6/17/2019
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