Cardiac Troponin I antibody LS-C58316 is an unconjugated mouse monoclonal antibody to human Cardiac Troponin I (TNNI3). Validated for ELISA and WB.
Human TNNI3 / Cardiac Troponin I
(tested or 100% immunogen sequence identity)
Dephosphorylated form of human cardiac Troponin I.
Recognizes the dephosphorylated form of human cardiac Troponin I (cTnI). Troponin I is a 24kD subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts. Recognizes the dephosphorylated form of cTnI. Recognizes amino acid residues 20-24 on cTnI. The antibody will recognize free dephosphorylated cTnI or dephosphorylated cTnI in complex with other troponin components. Does not cross react with phosphorylated cTnI or with skeletal TnI.
PBS, pH 7.4, 0.09% Sodium Azide
Short term: -20°C; Long term: -20°C.
For research use only.
About TNNI3 / Cardiac Troponin I