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Catalog Number Size Price
LS-C745263-100 100 µl (1 mg/ml) $567 
TNF Alpha Antibody - Western Blot of Mouse TNFa Antibody. Lane 1: Mouse TNFa. Load: 50 ng per lane. Primary antibody: Mouse TNFa antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Mouse TNFa.

Polyclonal Rabbit anti‑Mouse TNF Alpha Antibody (IHC, WB) LS‑C745263

Polyclonal Rabbit anti‑Mouse TNF Alpha Antibody (IHC, WB) LS‑C745263

Antibody:
TNF Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, WB, IP, ELISA, Neut, RIA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C745263-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNF Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, WB, IP, ELISA, Neut, RIA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
TNF Alpha antibody LS-C745263 is an unconjugated rabbit polyclonal antibody to mouse TNF Alpha. Validated for ELISA, IHC, IP, Neut, RIA and WB.
Target
Mouse TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This antibody was produced from whole rabbit serum prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.
Specificity
The antibody is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFß (lymphotoxin).
Applications
  • IHC
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • ELISA (1:1000 - 1:5000)
  • Neutralization
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024