Mouse TNF Alpha

Rabbit

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.

The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

• IHC
• Western blot (1:500 - 1:2000)
• Immunoprecipitation
• ELISA (1:1000 - 1:5000)
• Neutralization
• Radioimmunoassay

This antiserum against Mouse TNF alpha has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. It recognizes the 17000 MW TNF alpha. Reactivity in other immunoassays is unknown.

Antiserum

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01375 NCBI: NM_000594 NP_000585.2