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Catalog Number Size Price
LS-C745236-100 100 µl (1 mg/ml) $485 
TNF Alpha Antibody - Immunohistochemistry using the polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
TNF Alpha Antibody - Fluorescent immunohistochemistry showing staining of human colon by Anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor 680 goat polyclonal secondary antibody was used diluted 1:5000.
TNF Alpha Antibody - Western blot using the Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gt-a-Rb 611-143-122 added at 1:20,000 in MB-070 30 min RT.
TNF Alpha Antibody - Western Blot of rabbit anti-TNF Alpha antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: TNF Alpha antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 26kDa for TNF Alpha.
TNF Alpha Antibody - Western blot of Anti-Human TNF-a (RABBIT) Antibody. Lane 1: 250ng human recombinant TNF. Lane 2: 500ng human recombinant TNF. Lane 3: 1000ng human recombinant TNF. Membrane blocked in Blotto p/n B501-0500 for 30 min RT. Primary antibody: Rb-a-TNF alpha added at 1:1000 in overnight at 4°C. Secondary Antibody : Gt-a-Rb HRP p/n 611-103-122 added at 1:20,000 in 5% Blotto for 30 min RT. (5 second exposure).
TNF Alpha Antibody - Immunohistochemistry using the polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
TNF Alpha Antibody - Fluorescent immunohistochemistry showing staining of human colon by Anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor 680 goat polyclonal secondary antibody was used diluted 1:5000.
TNF Alpha Antibody - Western blot using the Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gt-a-Rb 611-143-122 added at 1:20,000 in MB-070 30 min RT.
TNF Alpha Antibody - Western Blot of rabbit anti-TNF Alpha antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: TNF Alpha antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 26kDa for TNF Alpha.
TNF Alpha Antibody - Western blot of Anti-Human TNF-a (RABBIT) Antibody. Lane 1: 250ng human recombinant TNF. Lane 2: 500ng human recombinant TNF. Lane 3: 1000ng human recombinant TNF. Membrane blocked in Blotto p/n B501-0500 for 30 min RT. Primary antibody: Rb-a-TNF alpha added at 1:1000 in overnight at 4°C. Secondary Antibody : Gt-a-Rb HRP p/n 611-103-122 added at 1:20,000 in 5% Blotto for 30 min RT. (5 second exposure).
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Polyclonal Rabbit anti‑Human TNF Alpha Antibody (IHC, IF, WB) LS‑C745236

Polyclonal Rabbit anti‑Human TNF Alpha Antibody (IHC, IF, WB) LS‑C745236

Antibody:
TNF Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-C745236-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
TNF Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TNF Alpha antibody LS-C745236 is an unconjugated rabbit polyclonal antibody to human TNF Alpha. Validated for ELISA, IF, IHC, Neut and WB.
Target
Human TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFa.
Specificity
This antibody is primarily directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:1000 - 1:5000)
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2021