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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TMEM5 Antibody (Extracellular Domain) IHC‑plus™ LS‑A3043
TMEM5 antibody LS-A3043 is an unconjugated rabbit polyclonal antibody to human TMEM5. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A3043-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular TMEM5 Products

Anti-TMEM5 antibody LS-A3048 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin
Anti-TMEM5 antibody LS-A3042 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Anti-TMEM5 antibody LS-A3042 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin

Product Description

TMEM5 antibody LS-A3043 is an unconjugated rabbit polyclonal antibody to human TMEM5. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human TMEM5
Synonyms
TMEM5, HP10481, Transmembrane protein 5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TMEM5 antibody was raised against synthetic 17 amino acid peptide from extracellular domain of human TMEM5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Gorilla, Marmoset, Elephant (94%); Rat, Hamster, Horse (88%); Dog, Panda, Pig (82%).
Blocking Peptide
LS-E28675 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A3043. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Extracellular Domain
Specificity
Human TMEM5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A3043 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A3043 was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TMEM5
TMEM5 encodes a type II transmembrane protein that is thought to have glycosyltransferase function. Mutations in this gene result in cobblestone lissencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Q9Y2B1 NM_014254 NP_055069.1


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Immunohistochemistry

Anti-TMEM5 antibody LS-A3043 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TMEM5 antibody LS-A3043 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TMEM5 antibody LS-A3043 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TMEM5 antibody LS-A3043 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TMEM5 antibody LS-A3043 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TMEM5 antibody LS-A3043 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TMEM5 antibody LS-A3043 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TMEM5 antibody LS-A3043 IHC of human ovary, oocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/15/2018

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