TLR8 antibody LS-B430 is an unconjugated rabbit polyclonal antibody to TLR8 from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:2000)
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TLR8 antibody was raised against synthetic peptide from human TLR8.
An internal region of human TLR8 protein.
Immunohistochemistry: LS-B430 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B430 was determined to be 5 ug/ml.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.