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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TLR8 Antibody (Internal) IHC‑plus™ LS‑B430
Note: This antibody replaces LS-C19085
TLR8 antibody LS-B430 is an unconjugated rabbit polyclonal antibody to TLR8 from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B430-50
50 µg (1 mg/ml)
$425
Description
TLR8 antibody LS-B430 is an unconjugated rabbit polyclonal antibody to TLR8 from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TLR8
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TLR8 antibody was raised against synthetic peptide from human TLR8.
Epitope
Internal
Specificity
An internal region of human TLR8 protein.
Usage
Immunohistochemistry: LS-B430 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B430 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
About TLR8
Q9NR97 NM_138636 NP_619542.1

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Immunohistochemistry

Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.
Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.

Immunohistochemistry

Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.
Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.

Immunohistochemistry

Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.
Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.

Immunohistochemistry

Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.
Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.

Requested From: United States
Date Requested: 12/10/2018
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