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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TLR8 Antibody IHC-plus™ LS-B1421

Note: This antibody replaces LS-C359, LS-C43045, LS-C43044
TLR8 antibody LS-B1421 is an unconjugated rabbit polyclonal antibody to TLR8 from human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1421-0.05
0.05 mg (1 mg/ml)
$425
Description
TLR8 antibody LS-B1421 is an unconjugated rabbit polyclonal antibody to TLR8 from human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TLR8
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (0.5 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TLR8 antibody was raised against a peptide corresponding to 16 amino acids near the middle of human TLR8. The immunogen is located within amino acids 790 - 840 of TLR8.
Blocking Peptide
LS-E30059 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B1421. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
TLR8 antibody can be used for detection of TLR8 by Western blot at 0.5 to 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 112 kDa Observed: 107 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About TLR8
Q9NR97 NM_138636 NP_619542.1

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Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Antibody
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Intracellular flow analysis of TLR8 in Ramos cells using 2 ug of TLR8 Antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control, ; red represents anti-TLR8 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: Flow Cytometry

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Images

Immunohistochemistry

Anti-TLR8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 ug/ml.
Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 ug/ml.

Immunohistochemistry

Anti-TLR8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 ug/ml.
Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 ug/ml.

Immunohistochemistry

Anti-TLR8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 ug/ml.
Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 ug/ml.

Immunohistochemistry

Anti-TLR8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 ug/ml.
Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 ug/ml.

Requested From: United States
Date Requested: 3/21/2019
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