TLR4 antibody LS-C755 is an unconjugated rabbit polyclonal antibody to mouse TLR4. Validated for WB.
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (1 - 3 µg/ml)
TLR4 antibody was raised against this antibody was developed against a synthetic peptide corresponding to amino acids 39-56 (CMDQKLSKVPDDIPSSTK) of mouse TLR4, GenBank noCAD83014.1. Percent identity by BLAST analysis: Mouse (100%).
This was tested against partial recombinant mouse TLR4 (extracellular portion), and a 75-80 kD band was observed. Full-length TLR4 is observed at approximately 90 kD. 2. Zager et al (2006, Fig. 10) observed TLR4 as an ~95 kD doublet which is thought to represent different degrees of glycosylation. See Product Citation (Renal Physiol 291: F546-F556) for additional information. 3. Presumptive TLR4 30 and 60 kD cleavage fragments are described in Zagar et al 2006.
PBS, 0.05% Sodium Azide, 0.05% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.