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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TLR3 Antibody (aa135‑150) LS‑C754
Note: This antibody replaces LS-C90551, LS-C90552, LS-C10688, LS-C10693
TLR3 antibody LS-C754 is an unconjugated rabbit polyclonal antibody to mouse TLR3. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C754-100
100 µg (0.5 mg/ml)
$335

Popular TLR3 Products

Anti-TLR3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B687 concentration 5 ug/ml.
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Anti-TLR3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4866 concentration 10 ug/ml.
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IHC of human gut lumen (longitudinal section, transverse region) using antibody at 10 ug/ml.
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Species: Human
Applications: ICC, Immunoprecipitation, Flow Cytometry, Blocking
Antibody
Species: Human, Mouse, Dog
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay

Product Description

TLR3 antibody LS-C754 is an unconjugated rabbit polyclonal antibody to mouse TLR3. Validated for Flow, IHC and WB.

Specifications

Target
Mouse TLR3
Synonyms
TLR3, CD283, Toll-like receptor 3, CD283 antigen, IIAE2
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Western blot (1 - 3 µg/ml)
  • Flow Cytometry (1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
TLR3 antibody was raised against a mix of synthetic peptides corresponding to amino acids 135-150 (SIHKIKSNPFKNQKNL), 828-844 (CRRFKVHHAVQQAIEQN), and 876-891 (CILNWPVQKERINAFH) of mouse TLR3. Percent identity by BLAST analysis: Mouse (100%); Rat, Elephant (88%); Marmoset, Rabbit (81%).
Epitope
aa135-150
Specificity
A mix of synthetic peptides corresponding to amino acids 135-150 (SIHKIKSNPFKNQKNL), 828-844 (CRRFKVHHAVQQAIEQN), and 876-891 (CILNWPVQKERINAFH) of mouse TLR3.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TLR3
TLR3 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. O15455 NM_003265 NP_003256.1


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Images

Western blot

Western blot of TLR3 in 15 ug/lane mouse spleen tissue lysate.
Western blot of TLR3 in 15 ug/lane mouse spleen tissue lysate.

Flow Cytometry

Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using antibody at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control ; red represents anti-TLR3 antibody.
Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using antibody at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control ; red represents anti-TLR3 antibody.

Western blot

Western blot of TLR3 in 15 ug/lane mouse spleen tissue lysate.
Western blot of TLR3 in 15 ug/lane mouse spleen tissue lysate.

Flow Cytometry

Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using antibody at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control ; red represents anti-TLR3 antibody.
Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using antibody at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control ; red represents anti-TLR3 antibody.

Western blot

Western blot of TLR3 in 15 ug/lane mouse spleen tissue lysate.
Western blot of TLR3 in 15 ug/lane mouse spleen tissue lysate.

Flow Cytometry

Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using antibody at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control ; red represents anti-TLR3 antibody.
Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using antibody at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control ; red represents anti-TLR3 antibody.

Western blot

Western blot of TLR3 in 15 ug/lane mouse spleen tissue lysate.
Western blot of TLR3 in 15 ug/lane mouse spleen tissue lysate.

Flow Cytometry

Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using antibody at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control ; red represents anti-TLR3 antibody.
Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using antibody at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control ; red represents anti-TLR3 antibody.

Requested From: United States
Date Requested: 10/17/2018

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