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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C18924-100 100 µg (0.6 mg/ml) $425 
TLK1 Antibody - Anti-Tlk-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Tlk-1 antibody shows detection of a band ~64 kD corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal TLK1 Antibody (aa730‑755, WB) LS‑C18924

Polyclonal TLK1 Antibody (aa730‑755, WB) LS‑C18924

Antibody:
Rabbit Polyclonal to Human TLK1
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee, Orangutan, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18924-100
100 µg
0.6 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TLK1
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee, Orangutan, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
TLK1 antibody LS-C18924 is an unconjugated rabbit polyclonal antibody to TLK1 from human, chimpanzee, dog and other species. Validated for ELISA and WB.
Target
Human TLK1
Synonyms
TLK1 | KIAA0137 | Tousled-like kinase 1 | Snare kinase snak | PKU-beta | SNARE protein kinase SNAK
Host
Rabbit
Reactivity
Human, Chimpanzee, Orangutan, Dog (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Orangutan, Mouse, Rat, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 730-755 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.
Epitope
aa730-755
Specificity
This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein. The antibody will react with all isoforms of Tlk-1. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, and dog sources based on 100% homology for the immunogen sequence. Cross reactivity with Tlk-1 protein from mouse, rat and chicken may occur as this sequence only varies by one amino acid residue (93% homology). Cross reactivity with Tlk-1 homologues from other sources has not been determined.
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kD, 89 kD or 64 kD in size corresponding to isoforms of TLk-1 by western blotting in the appropriate cell lysate or extract. This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLK1
Q9UKI8 NM_012290 NP_036422.3

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Requested From: United States
Date Requested: 2/26/2020