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Catalog Number Size Price
LS-C18924-100 100 µg (0.6 mg/ml) $498 
TLK1 Antibody - Anti-Tlk-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Tlk-1 antibody shows detection of a band ~64 kD corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Human TLK1 Antibody (aa730‑755, WB) LS‑C18924

Polyclonal Rabbit anti‑Human TLK1 Antibody (aa730‑755, WB) LS‑C18924

Antibody:
TLK1 Rabbit anti-Human Polyclonal (aa730-755) Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee, Orangutan, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18924-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TLK1 Rabbit anti-Human Polyclonal (aa730-755) Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee, Orangutan, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
TLK1 antibody LS-C18924 is an unconjugated rabbit polyclonal antibody to TLK1 (aa730-755) from human. It is reactive with human, chimpanzee, dog and other species. Validated for ELISA and WB.
Target
Human TLK1
Synonyms
TLK1 | KIAA0137 | Tousled-like kinase 1 | Snare kinase snak | PKU-beta | SNARE protein kinase SNAK
Host
Rabbit
Reactivity
Human, Chimpanzee, Orangutan, Dog (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Orangutan, Mouse, Rat, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 730-755 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.
Epitope
aa730-755
Specificity
This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein. The antibody will react with all isoforms of Tlk-1. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, and dog sources based on 100% homology for the immunogen sequence. Cross reactivity with Tlk-1 protein from mouse, rat and chicken may occur as this sequence only varies by one amino acid residue (93% homology). Cross reactivity with Tlk-1 homologues from other sources has not been determined.
Applications
  • Western blot (1:200 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kD, 89 kD or 64 kD in size corresponding to isoforms of TLk-1 by western blotting in the appropriate cell lysate or extract. This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLK1
Q9UKI8 NM_012290 NP_036422.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/14/2022