Human TJP2 / ZO2 / ZO-2

Rabbit

Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the C-Terminus of human or canine ZO-2.

C-Terminus

Recognizes the ~130-160kD human ZO-2 protein, isoforms A or C. A band of unknown origin lower than 75kD may be observed in certain lysates.

• IHC
• IHC - Paraffin (5 - 10 µg/ml)
• Immunofluorescence (2.5 µg/ml)
• Western blot (1 - 3 µg/ml)

Immunohistochemistry: LS-B2185 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2185 was determined to be 5-10 ug/ml. Immunofluorescence: 2.5 ug/ml. Cells need to be fixed with ice-cold ethanol for 30 minutes on ice prior to assays. Positive control: Western Blot: CaCO2 human colorectal adenocarcinoma, MDCK II canine kidney, KNRK rat kidney, and TMCK-1 mouse kidney cell lysates.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UDY2 NCBI: NM_004817 NP_004808.2