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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TJP2 / ZO2 / ZO-2 Antibody (AP) LS-C512157

ZO-2 antibody LS-C512157 is an AP-conjugated mouse monoclonal antibody to ZO-2 (TJP2 / ZO2) from human and dog. Validated for ELISA, IF, IP and WB.
100 µl
ZO-2 antibody LS-C512157 is an AP-conjugated mouse monoclonal antibody to ZO-2 (TJP2 / ZO2) from human and dog. Validated for ELISA, IF, IP and WB.
Human TJP2 / ZO2 / ZO-2
Human, Dog (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Alkaline Phosphatase. Also available Unconjugated or conjugated with APC, Biotin, FITC, HRP, PE.
Protein A purified
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Synthetic peptide corresponding to internal amino acids of the human ZO-2 protein.
Specific for human and canine ZO-2. On Western blots, it identifies a band at 160kD. Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates.
PBS, pH 7.2, No preservatives added
Store at 4°C.
For research use only.
About TJP2 / ZO2 / ZO-2
Q9UDY2 NM_004817 NP_004808.2

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Immunofluorescence analysis of ZO2/TJP2 Antibody was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO2/TJP2 Antibody at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing membrane localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
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Requested From: United States
Date Requested: 4/22/2019
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