Target
Human THOP1 / Thimet Oligopeptidase
Synonyms
THOP1 | Endopeptidase 24.15 | Endopeptidase 3.4.24.15 | EP24.15 | MEPD_HUMAN | Metalloendopeptidase 3.4.24.15 | MP78 | Thimet oligopeptidase | Thimet oligopeptidase 1 | TOP
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
E. coli-derived rhTHOP1 (aa 1-689).
Specificity
Selected for its ability to recognize human THOP1 in direct ELISAs and Western Blots. In Western Blots, this antibody shows less than 5% cross-reactivity with rhNeurolysin.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Immunoprecipitation
- ELISA
Usage
Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human THOP1. The detection limit for rhTHOP1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhTHOP1 from conditioned media of transfected NS0 cells. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human THOP1. The detection limit for rhTHOP1 is approximately 0.2 ng/well.
Reconstitution
Reconstitute in deionized water
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THOP1 / Thimet Oligopeptidase