Target
Mouse TFPI2
Synonyms
TFPI2 | Placental protein 5 | TFPI-2 | PP5 | REF1
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rmTFPI-2.
Specificity
Detects rmTFPI-2 in direct ELISAs and Western Blots. Shows no cross-reactivity with rmTFPI or rhTFPI-2.
Applications
- Western blot
- Immunoprecipitation
- ELISA
Usage
Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect mouse TFPI-2. Using a colorimetric detection system, the detection limit for rmTFPI-2 is approximately 10 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: This antibody was used at a concentration of 25 ug/ml to immunoprecipitate rmTFPI-2 from conditioned cell culture medium. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse TFPI-2. The detection limit for rmTFPI-2 is approximately 5 ng/well.
Presentation
Supplied as a powder lyophilized from a solution containing PBS with 5% trehalose.
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Store lyophilized powder at 4°C for short term only, after reconstitution, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee