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Catalog Number Size Price
LS-C744600-25 25 µl (90 mg/ml) $304 
Tetanus Toxin Fragment C Antibody - Western blot using the Anti-tetanus toxin C antibody shows detection of a protein band at ~ 52 kDa corresponding to full length 6XHIS-TTFC fusion protein (arrowhead). Lanes 1-5 contain 10 ng, 5 ng, 2.5ng, 1.25 ng and 0.0625 ng protein respectively. MW markers are shown for size comparison. After blocking, the membrane was probed with the primary antibody diluted to 1:100,000 followed by reaction with a 1:20,000 dilution of HRP conjugated donkey-anti-Rabbit IgG [H&L].
Tetanus Toxin Fragment C Antibody - Western blot using the Anti-tetanus toxin C antibody shows detection of a protein band at 52 kDa corresponding to full length 6X HIS-TTFC fusion protein (arrowhead). Lane 1 shows no detection from non-specific antisera; Lane 2 shows detection of anti-TTFC. After blocking in 1% BSA, the membrane was probed with the primary antisera diluted to 1:1,000 in PBS followed by reaction with HRP conjugated Goat anti-Rabbit at 1:20,000 dilution.
Tetanus Toxin Fragment C Antibody - Structure of Tetanus toxin. Fragment C is the non-toxic portion of the heavy chain that specifically binds neurons (ganglioside binding domain).
Tetanus Toxin Fragment C Antibody - Western blot using the Anti-tetanus toxin C antibody shows detection of a protein band at ~ 52 kDa corresponding to full length 6XHIS-TTFC fusion protein (arrowhead). Lanes 1-5 contain 10 ng, 5 ng, 2.5ng, 1.25 ng and 0.0625 ng protein respectively. MW markers are shown for size comparison. After blocking, the membrane was probed with the primary antibody diluted to 1:100,000 followed by reaction with a 1:20,000 dilution of HRP conjugated donkey-anti-Rabbit IgG [H&L].
Tetanus Toxin Fragment C Antibody - Western blot using the Anti-tetanus toxin C antibody shows detection of a protein band at 52 kDa corresponding to full length 6X HIS-TTFC fusion protein (arrowhead). Lane 1 shows no detection from non-specific antisera; Lane 2 shows detection of anti-TTFC. After blocking in 1% BSA, the membrane was probed with the primary antisera diluted to 1:1,000 in PBS followed by reaction with HRP conjugated Goat anti-Rabbit at 1:20,000 dilution.
Tetanus Toxin Fragment C Antibody - Structure of Tetanus toxin. Fragment C is the non-toxic portion of the heavy chain that specifically binds neurons (ganglioside binding domain).
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Polyclonal Rabbit anti‑Clostridium tetani Tetanus Toxin Fragment C Antibody (WB) LS‑C744600

Polyclonal Rabbit anti‑Clostridium tetani Tetanus Toxin Fragment C Antibody (WB) LS‑C744600

Antibody:
Tetanus Toxin Fragment C Rabbit anti-Clostridium tetani Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Clostridium tetani
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C744600-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Tetanus Toxin Fragment C Rabbit anti-Clostridium tetani Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Clostridium tetani
Format:
Unconjugated, Unmodified

Specifications

Description
Tetanus Toxin Fragment C antibody LS-C744600 is an unconjugated rabbit polyclonal antibody to clostridium tetani Tetanus Toxin Fragment C. Validated for ELISA and WB.
Target
Clostridium tetani Tetanus Toxin Fragment C
Host
Rabbit
Reactivity
Clostridium tetani (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged TTFC fusion protein expressed in E. coli corresponding to full length TTFC.
Specificity
This antibody is directed against the C fragment of Tetanus toxin derived from Clostridium tetani. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross reactivity with Clostridium tetani. Cross-reactivity with TTFC from other sources have not been determined.
Applications
  • Western blot (1:1000 - 1:40000)
  • ELISA (1:50000 - 1:250000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

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Requested From: United States
Date Requested: 4/24/2024