Human TERF2 / TRF2

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

4A794.15

Unconjugated

Protein G purified

Azide-free

Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).

Human TERF2 / TRF2. The recognizes distinct doublet bands of approximate size 66 kD corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines.

• IHC
• IHC - Paraffin (1:10 - 1:500)
• IHC - Frozen (5 µg/ml)
• ICC (1:10 - 1:500)
• Immunofluorescence (1:10 - 1:500)
• Western blot (2 - 4 µg/ml)
• Immunoprecipitation (2 µg/10E6 cells)
• Flow Cytometry (0.1 µg/10E6 cells)
• ELISA (2 µg/ml)
• Chromatin Immunoprecipitation (1:10 - 1:500)

TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. However, observed molecular weights could vary depending on molecular weight standards used and gel conditions. 2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. 3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information. 4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS. No stabilizing proteins added.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q15554 NCBI: NM_005652 NP_005643.2