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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C157160-400 400 µl $325 
TCF25 Antibody - Fluorescent image of A549 cell stained with TCF25 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). TCF25 immunoreactivity is localized to Cytoplasm significantly.
TCF25 Antibody - TCF25 Antibody western blot of CEM cell line lysates (35 ug/lane). The TCF25 antibody detected the TCF25 protein (arrow).
TCF25 Antibody - Fluorescent image of A549 cell stained with TCF25 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). TCF25 immunoreactivity is localized to Cytoplasm significantly.
TCF25 Antibody - TCF25 Antibody western blot of CEM cell line lysates (35 ug/lane). The TCF25 antibody detected the TCF25 protein (arrow).
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Polyclonal Rabbit anti‑Human TCF25 Antibody (aa186‑215, IF, WB) LS‑C157160

Polyclonal Rabbit anti‑Human TCF25 Antibody (aa186‑215, IF, WB) LS‑C157160

Antibody:
Rabbit Polyclonal to Human TCF25
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C157160-400
400 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human TCF25
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TCF25 antibody LS-C157160 is an unconjugated rabbit polyclonal antibody to human TCF25 (aa186-215). Validated for IF and WB.
Target
Human TCF25
Synonyms
TCF25 | FKSG26 | Hulp1 | Nuclear localized protein 1 | NULP1 | PRO2620 | TCF-25 | HKIAA1049 | KIAA1049 | Transcription factor 25
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Blocking Peptide
LS-E4898 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-C157160. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa186-215
Specificity
This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TCF25
Q9BQ70 NM_014972 NP_055787.1

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Requested From: United States
Date Requested: 6/6/2020