Human EPCAM

Mouse

Human (tested or 100% immunogen sequence identity)

Rat

IgG1,k Recombinant Monoclonal

rMOC-31

Unconjugated

Protein A/G purified

Unmodified. Also available Azide-free, Carrier-free.

Recombinant full-length human EpCAM protein. Antigen Molecular Weight: 40-43 kDa

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of EpCAM. EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (EpCAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

• IHC - Paraffin (0.5 - 1 µg/ml)

Immunofluorescence (1-2 ug/ml) - Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 min at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16422 NCBI: NM_002354 NP_002345.2