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Antibodies > Sulfatase 1 / SULF1 > LS-C757667
 
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Catalog Number Size Price
LS-C757667-200 200 µl $393 
Sulfatase 1 / SULF1 Antibody - Anti-SULF1 Antibody (C-Term) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Sulfatase 1 / SULF1 Antibody - Overlay histogram showing U-251 MG cells stained with SULF1 Antibody (C-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (SULF1 Antibody (C-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Sulfatase 1 / SULF1 Antibody - Anti-SULF1 Antibody (C-Term) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Sulfatase 1 / SULF1 Antibody - Overlay histogram showing U-251 MG cells stained with SULF1 Antibody (C-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (SULF1 Antibody (C-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human Sulfatase 1 / SULF1 Antibody (C‑Terminus, WB) LS‑C757667

Polyclonal Rabbit anti‑Human Sulfatase 1 / SULF1 Antibody (C‑Terminus, WB) LS‑C757667

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Antibody:
Sulfatase 1 / SULF1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757667-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Sulfatase 1 / SULF1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SULF1 antibody LS-C757667 is an unconjugated rabbit polyclonal antibody to human SULF1 (Sulfatase 1) (C-Terminus). Validated for ELISA, Flow and WB.
Target
Human Sulfatase 1 / SULF1
Synonyms
SULF1 | Extracellular sulfatase Sulf-1 | HSULF-1 | KIAA1077 | Sulfatase FP | SULF-1 | Sulfatase 1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This SULF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 704-738 amino acids from human SULF1.
Epitope
C-Terminus
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 101 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Sulfatase 1 / SULF1
Q8IWU6 AB029000 BAA83029.1

Publications (0)

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Featured Products

Sulfatase 1 / SULF1 Antibody - Immunohistochemistry of paraffin-embedded human lung tissue at dilution 1:100
Sulfatase 1 / SULF1 Antibody LS‑C679684
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Sulfatase 1 / SULF1 Antibody - Western blot of recombinant Sulfatase 1 / SULF1. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sulfatase 1 / SULF1 Antibody (aa609‑871) LS‑C296521
Species: Human
Applications: Western blot
Sulfatase 1 / SULF1 Antibody - Western blot of recombinant Sulfatase 1 / SULF1. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sulfatase 1 / SULF1 Antibody (aa609‑871, APC) LS‑C719003
Species: Human
Applications: Western blot
Sulfatase 1 / SULF1 Antibody - Western blot of recombinant Sulfatase 1 / SULF1. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sulfatase 1 / SULF1 Antibody (aa609‑871, Cy3) LS‑C704811
Species: Human
Applications: Western blot
Sulfatase 1 / SULF1 ELISA Kit
Human Sulfatase 1 / SULF1 (Sandwich ELISA) ELISA Kit - LS-F66757
Reactivity: Human
Range: 78-5000 pg/ml
Sulfatase 1 / SULF1 ELISA Kit
Human Sulfatase 1 / SULF1 (Sandwich ELISA) ELISA Kit - LS-F54622
Reactivity: Human
Range: 7.8-500 pg/ml
See all Sulfatase 1 / SULF1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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