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Catalog Number Size Price
LS-C744774-25 25 µl (1 mg/ml) $304 
Sucrose Phosphorylase Antibody - Goat anti Sucrose Phosphorylase antibody was used to detect purified Sucrose Phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System

Polyclonal Goat anti‑E. coli Sucrose Phosphorylase Antibody (Liquid, Biotin, WB) LS‑C744774

Polyclonal Goat anti‑E. coli Sucrose Phosphorylase Antibody (Liquid, Biotin, WB) LS‑C744774

Antibody:
Sucrose Phosphorylase Goat anti-E. coli Polyclonal (Liquid) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
E. coli
Format:
Biotin, Liquid
Price
Catalog Number
$304
LS-C744774-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Sucrose Phosphorylase Goat anti-E. coli Polyclonal (Liquid) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
E. coli
Format:
Biotin, Liquid

Specifications

Description
Sucrose Phosphorylase antibody LS-C744774 is a biotin-conjugated goat polyclonal antibody to e. coli Sucrose Phosphorylase. Validated for ELISA, IP and WB.
Target
E. coli Sucrose Phosphorylase
Host
Goat
Reactivity
E. coli (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Sucrose Phosphorylase [E.coli]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli]. Cross reactivity against Sucrose Phosphorylase from other sources is unknown.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024