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Catalog Number Size Price
LS-C744830-1 1 mg (1 mg/ml) $1,934 
STAT5A Antibody - Immunohistochemistry using Anti-STAT5 pY694 monoclonal antibody shows detection of phosphorylated STAT5 pY694 in human breast tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
STAT5A Antibody - Anti-STAT5 pY694 (MOUSE) Monoclonal Antibody detecting STAT5 in 3T3 cells (immunofluorescent STED microscopy). Red represents STAT5 pY694 protein. Green represents tubulin.
STAT5A Antibody - Western blot using the Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4° C. For detection DyLight 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature followed by visualization using a VersaDoc MP 4000 imaging system (Bio-Rad).
STAT5A Antibody - Immunohistochemistry using Anti-STAT5 pY694 monoclonal antibody shows detection of phosphorylated STAT5 pY694 in human breast tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
STAT5A Antibody - Anti-STAT5 pY694 (MOUSE) Monoclonal Antibody detecting STAT5 in 3T3 cells (immunofluorescent STED microscopy). Red represents STAT5 pY694 protein. Green represents tubulin.
STAT5A Antibody - Western blot using the Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4° C. For detection DyLight 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature followed by visualization using a VersaDoc MP 4000 imaging system (Bio-Rad).
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Monoclonal Mouse anti‑Mouse STAT5A Antibody (clone 5F6.F1, phospho‑Tyr694, IHC, IF, WB) LS‑C744830

Monoclonal Mouse anti‑Mouse STAT5A Antibody (clone 5F6.F1, phospho‑Tyr694, IHC, IF, WB) LS‑C744830

Antibody:
STAT5A Mouse anti-Mouse Monoclonal (pTyr694) (5F6.F1) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$1,934
LS-C744830-1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
STAT5A Mouse anti-Mouse Monoclonal (pTyr694) (5F6.F1) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
STAT5A antibody LS-C744830 is an unconjugated mouse monoclonal antibody to STAT5A (pTyr694) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Target
Mouse STAT5A
Synonyms
STAT5A | MGF | STAT5
Host
Mouse
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Predicted
Human, Rat (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
5F6.F1
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Phospho STAT5 pY694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Epitope
pTyr694
Specificity
This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
Applications
  • IHC (20 - 40 µg/ml)
  • Immunofluorescence (1:50 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About STAT5A
P42229 NM_003152 NP_003143.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021