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Catalog Number Size Price
LS-C745353-25 25 µl (0.9 mg/ml) $275 
STAT3 Antibody - Immunohistochemistry of rabbit anti STAT3 pY705 Antibody. Tissue: Human Colon at 40X in colon tissue at pH 9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STAT3 pY705 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: STAT3 phospho Y705 is nuclear and cytoplasmic. Staining: STAT3 (pTyr705) as precipitated red signal with hematoxylin purple nuclear counterstain.
STAT3 Antibody - Western Blot of rabbit anti-Stat3 pY705 antibody. Lane 1: Jurkat lysate. Lane 2: Jurkat lysate IFN-a treatment. Load: 35 µg per lane. Primary antibody: STAT3 pY705 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 88 kDa, ~90kDa for STAT3py705. Other band(s): STAT 3 pY705 splice variants and isoforms.
STAT3 Antibody - Immunohistochemistry of rabbit anti STAT3 pY705 Antibody. Tissue: Human Colon at 40X in colon tissue at pH 9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STAT3 pY705 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: STAT3 phospho Y705 is nuclear and cytoplasmic. Staining: STAT3 (pTyr705) as precipitated red signal with hematoxylin purple nuclear counterstain.
STAT3 Antibody - Western Blot of rabbit anti-Stat3 pY705 antibody. Lane 1: Jurkat lysate. Lane 2: Jurkat lysate IFN-a treatment. Load: 35 µg per lane. Primary antibody: STAT3 pY705 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 88 kDa, ~90kDa for STAT3py705. Other band(s): STAT 3 pY705 splice variants and isoforms.
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Polyclonal Rabbit anti‑Human STAT3 Antibody (phospho‑Tyr705, IHC, WB) LS‑C745353

Polyclonal Rabbit anti‑Human STAT3 Antibody (phospho‑Tyr705, IHC, WB) LS‑C745353

Antibody:
STAT3 Rabbit anti-Human Polyclonal (pTyr705) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745353-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
STAT3 Rabbit anti-Human Polyclonal (pTyr705) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
STAT3 antibody LS-C745353 is an unconjugated rabbit polyclonal antibody to human STAT3 (pTyr705). Validated for ELISA, IHC and WB.
Target
Human STAT3
Synonyms
STAT3 | Acute-phase response factor | APRF | DNA-binding protein APRF | HIES
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified Stat3 antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human Stat3.
Epitope
pTyr705
Specificity
A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • Western blot (1 µg/ml)
  • ELISA (1:60000 - 1:130000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About STAT3
P40763 NM_003150 NP_003141.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/15/2021