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Catalog Number Size Price
LS-C750557-20 20 µl $253 
LS-C750557-50 50 µl $306 
LS-C750557-100 100 µl $381 
LS-C750557-200 200 µl $489 
STAT3 Antibody - Western blot analysis of extracts of various cell lines, using Phospho-Stat3-S727 antibody at 1:1000 dilution or STAT3 antibody. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes or treated by UV at room temperature for 15-30 minutes. C6 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 20 minutes or treated by UV at room temperature for 15-30 minutes. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
STAT3 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Stat3-S727 pAb. Western blot was performed from the immunoprecipitate using Phospho-Stat3-S727 pAb at a dilition of 1:1000. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
STAT3 Antibody - Western blot analysis of extracts of various cell lines, using Phospho-Stat3-S727 antibody at 1:1000 dilution or STAT3 antibody. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes or treated by UV at room temperature for 15-30 minutes. C6 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 20 minutes or treated by UV at room temperature for 15-30 minutes. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
STAT3 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Stat3-S727 pAb. Western blot was performed from the immunoprecipitate using Phospho-Stat3-S727 pAb at a dilition of 1:1000. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
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Polyclonal Rabbit anti‑Human STAT3 Antibody (phospho‑Ser727, WB) LS‑C750557

Polyclonal Rabbit anti‑Human STAT3 Antibody (phospho‑Ser727, WB) LS‑C750557

Antibody:
STAT3 Rabbit anti-Human Polyclonal (pSer727) Antibody
Application:
WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C750557-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
STAT3 Rabbit anti-Human Polyclonal (pSer727) Antibody
Application:
WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
STAT3 antibody LS-C750557 is an unconjugated rabbit polyclonal antibody to STAT3 (pSer727) from human. It is reactive with human, mouse and rat. Validated for IP and WB.
Target
Human STAT3
Synonyms
STAT3 | Acute-phase response factor | APRF | DNA-binding protein APRF | HIES
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pSer727
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Usage
The predicted MW is 83kDa/87kDa/88kDa, while the observed MW by Western blot was 86kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About STAT3
P40763 NM_003150 NP_003141.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2021