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ST3GAL1 Antibody (aa254‑303) LS‑C80720 Best Seller
ST3GAL1 antibody LS-C80720 is an unconjugated rabbit polyclonal antibody to ST3GAL1 from human, rat, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C80720-50
50 µg
$365

Popular ST3GAL1 Products

IHC of paraffin-embedded serous OVCA using ST3GAL1 antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Western blot analysis of extracts of mouse liver, using ST3GAL1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 40s.
Species: Human, Mouse
Applications: Western blot
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Applications: Western blot
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Applications: Western blot

Product Description

ST3GAL1 antibody LS-C80720 is an unconjugated rabbit polyclonal antibody to ST3GAL1 from human, rat, bat and other species. Validated for WB.

Specifications

Target
Human ST3GAL1
Synonyms
ST3GAL1, Alpha 2,3-ST 1, Gal-NAc6S, Sialyltransferase 4A, SIAT4-A, SIATFL, ST3GalA, ST3GalIA,1, ST3O, SIAT4, ST3GalA.1, ST3GalI, SIAT4A, ST3Gal I, ST3GalIA
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
ST3GAL1 antibody was raised against synthetic peptide located between aa254-303 of human ST3GAL1 (Q11201, NP_003024). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Platypus (100%); Mouse, Opossum (92%); Lizard, Pufferfish (91%); Zebra finch (90%); Turkey, Chicken (85%); Stickleback (84%); Xenopus (83%).
Epitope
aa254-303
Specificity
Human ST3GAL1
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ST3GAL1
ST3GAL1 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Q11201 NM_003033 NP_003024.1


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Images

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/13/2018

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