Human ST14 / Matriptase

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

E. coli-derived rhMatriptase (aa 615-855).

aa615-855

Selected for its ability to recognize human Matriptase in direct ELISAs and Western Blots.

• Western blot (0.1 - 0.2 µg/ml)
• Flow Cytometry (25 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Flow Cytometry, ELISA and Western Blot. Flow Cytometry: 25 ug/ml; 10 ul labels 1-5x10^5 cells in a total reaction volume 200ul. Cells must first be fixed and permeabilized using 4% paraformaldehyde, 0.1% saponin in PBS. Tested using PC-3 cells. The binding of unlabeled antibodies may be visualized by adding an IgG (FITC). Direct ELISA: 0.5-1.0 ug/ml. Detection limit is ~0.5 ng/well. Western Blot: 0.1-0.2 ug/ml. Detection limit is ~1 ng/lane under non-reducing and reducing conditions.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute in 500 ul sterile PBS

Store at -20°C lyophilized. Once reconstituted: Store at -20°C. Aliquot to avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9Y5Y6 NCBI: NM_021978 NP_068813.1