Human SSEA-1 / Lewis x / CD15

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgM,k Monoclonal

FUT4/815 + BRA-4F1

Unconjugated

PEG purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Purified human neutrophils (FUT4/815) and Myelomonocytic leukemia cells (BRA-4F1) were used as the immunogen for the SSEA-1 antibody cocktail.

Human SSEA-1 / Lewis x / CD15

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Flow Cytometry

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. SSEA-1/CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, it is expressed in Reed-Sternberg cells and some epithelial cells. SSEA-1/CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. Optimal dilution of the SSEA-1 antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.