Human SRSF1 / SF2

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Recombinant SF2/ASF protein.

N-Terminus

Specific for the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. On Western blots, a band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences. SF2/ASF is highly conserved (e. g. mouse and human protein are 100% identical) so reactivity with other species is likely. Please note, however, that cross-reactivity in non-target species varies and cannot be guaranteed.

• IHC
• IHC - Paraffin (10 µg/ml)
• Immunofluorescence
• Western blot (1 - 3 µg/ml)

Immunohistochemistry: LS-B3263 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3263 was determined to be 10 ug/ml. Western Blot: 1-3 ug/ml. A band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q07955 NCBI: NM_006924 NP_008855.1