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Antibodies > SRC > LS-C96491
 
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Catalog Number Size Price
LS-C96491-400 400 µl $393 
SRC Antibody - Fluorescent image of A549 cell stained with SRC Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SRC primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SRC immunoreactivity is localized to Cytoplasm significantly.
SRC Antibody - FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src antibody was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degrees C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
SRC Antibody - The anti-Src antibody is used in Western blot to detect Src in Jurkat cell lysate.
SRC Antibody - Western blot of lysates from HT29, Jurkat cell line (from left to right), using SRC Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
SRC Antibody - Fluorescent image of A549 cell stained with SRC Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SRC primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SRC immunoreactivity is localized to Cytoplasm significantly.
SRC Antibody - FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src antibody was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degrees C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
SRC Antibody - The anti-Src antibody is used in Western blot to detect Src in Jurkat cell lysate.
SRC Antibody - Western blot of lysates from HT29, Jurkat cell line (from left to right), using SRC Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Monoclonal Mouse anti‑Human SRC Antibody (clone 17AT28, IF, WB) LS‑C96491

Monoclonal Mouse anti‑Human SRC Antibody (clone 17AT28, IF, WB) LS‑C96491

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
SRC Mouse anti-Human Monoclonal (17AT28) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C96491-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SRC Mouse anti-Human Monoclonal (17AT28) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SRC antibody LS-C96491 is an unconjugated mouse monoclonal antibody to human SRC. Validated for IF and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human SRC
Synonyms
SRC | ASV | Pp60c-src | Tyrosine kinase pp60c-src | Tyrosine-protein kinase SRC-1 | v-src | C-SRC | p60-Src | Proto-oncogene c-Src | SRC1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
17AT28
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:100 - 1:500)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SRC
P12931 NM_005417 NP_005408.1

LSBio Ratings

SRC Antibody (clone 17AT28) for IF/Immunofluorescence, WB/Western LS-C96491 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Interferon ß (IFN-ß) Production during the Double-stranded RNA (dsRNA) Response in Hepatocytes Involves Coordinated and Feedforward Signaling through Toll-like Receptor 3 (TLR3), RNA-dependent Protein Kinase (PKR), Inducible Nitric Oxide Synthase (iNOS), and Src Protein. Zhang L, Xiang W, Wang G, Yan Z, Zhu Z, Guo Z, Sengupta R, Chen AF, Loughran PA, Lu B, Wang Q, Billiar TR. The Journal of biological chemistry. 2016 291:15093-107. (WB; Human)
Full Text Article PubMed: 27226571 PMC: PMC4946926
Submit a Publication for LS-C96491

Customer Reviews (0)

Submit a review for LS-C96491

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Species: Human, Mouse, Rat
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
SRC Antibody - Immunohistochemical analysis of c-SRC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SRC Antibody (C‑Terminus) LS‑C352881
Species: Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
SRC Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
SRC Antibody LS‑C808630
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
SRC Protein - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Human SRC Protein (Recombinant 6His, N-terminus) (Gly2-Leu537) - LS-G26644
Source: E. coli
Tag: 6His, N-terminus
See all SRC Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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