Target
Xenopus SR Proteins
Reactivity
Xenopus, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
Mass-isolated nuclei from Xenopus Laevis oocytes.
Specificity
Detects the non-snRNP splicing factors termed SR proteins. Recognizes all members of the SR protein family. However, the epitope is phosphorylation-dependent so care must be taken to avoid dephosphorylation. Species cross-reactivity: Human and Xenopus.
Applications
- Immunofluorescence
- Western blot
- ELISA (0.1 - 1 µg/ml)
Usage
Suitable for use in Immunofluorescence, ELISA, Western Blot. Binding activity is confirmed by Western blotting of HeLa cell extracts and HeLa cell nuclear extracts. ELISA: 0.1-1.0 ug/ml. Immunofluorescence1,2:2-3 ug/ml. Western Blot1:0.4-10 ug/ml.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee