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Catalog Number Size Price
LS-C773154-100 100 µg (1 mg/ml) $477 
SPTBN4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) beta 4 Spectrin Antibody. (D) Composite.
SPTBN4 Antibody - Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.
SPTBN4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) beta 4 Spectrin Antibody. (D) Composite.
SPTBN4 Antibody - Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.
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Monoclonal Mouse anti‑Human SPTBN4 Antibody (clone S393‑2, RPE, IHC, IF, WB) LS‑C773154

Monoclonal Mouse anti‑Human SPTBN4 Antibody (clone S393‑2, RPE, IHC, IF, WB) LS‑C773154

Antibody:
SPTBN4 Mouse anti-Human Monoclonal (RPE) (S393-2) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Other formats:
Price
Catalog Number
$477
LS-C773154-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SPTBN4 Mouse anti-Human Monoclonal (RPE) (S393-2) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Other formats:

Specifications

Description
SPTBN4 antibody LS-C773154 is an RPE-conjugated mouse monoclonal antibody to SPTBN4 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human SPTBN4
Synonyms
SPTBN4 | Beta-IV spectrin | KIAA1642 | SPNB4 | SPTBN3 | QV | Spectrin beta chain, brain 3
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
S393-2
Conjugations
R. Phycoerythrin. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Fusion protein amino acids 1621-1832 (C-Terminus repeats 14 to 15) of human Beta4-spectrin
Specificity
Detects at about 200 kDa. Does not cross react with other Beta-spectrins
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPTBN4
Q9H254 NM_025213 NP_079489.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024