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Catalog Number Size Price
LS-C773154-100 100 µg (1 mg/ml) $395 
SPTBN4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) beta 4 Spectrin Antibody. (D) Composite.
SPTBN4 Antibody - Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.
SPTBN4 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) beta 4 Spectrin Antibody. (D) Composite.
SPTBN4 Antibody - Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.
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SPTBN4 Antibody (clone S393‑2, RPE) LS‑C773154

SPTBN4 Antibody (clone S393‑2, RPE) LS‑C773154

Antibody:
Mouse Monoclonal to Human SPTBN4
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Other formats:
Price
Catalog Number
$395 
LS-C773154-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human SPTBN4
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Other formats:

Specifications

Description
SPTBN4 antibody LS-C773154 is an RPE-conjugated mouse monoclonal antibody to SPTBN4 from human, mouse and rat. Validated for IF, IHC and WB.
Target
Human SPTBN4
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
S393-2
Conjugations
R. Phycoerythrin. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 565, Atto 594, Atto 633, Atto 655, Atto 680, Atto 700, Biotin, FITC, PE, Atto 594, PerCP, SA.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein amino acids 1621-1832 (C-Terminus repeats 14 to 15) of human Beta4-spectrin
Specificity
Detects at about 200 kDa. Does not cross react with other Beta-spectrins
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPTBN4
Q9H254 NM_025213 NP_079489.2

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Requested From: United States
Date Requested: 12/15/2019