SPRING / TRIM9 antibody was raised against synthetic peptide from C-Terminus of human TRIM9 (Q9C026, NP_443210). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Rabbit, Guinea pig, Turkey, Chicken (100%); Bat, Opossum, Zebra finch, Platypus (92%); Dog, Bovine, Pig, Xenopus (85%).
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
This antibody carries the LSBio 100% Guarantee.