Description
TRIM9 antibody LS-C80105 is an unconjugated rabbit polyclonal antibody to TRIM9 (SPRING) from human, mouse, rat and other species. Validated for WB.
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
FITC,
HRP.
Immunogen
SPRING / TRIM9 antibody was raised against synthetic peptide from C-Terminus of human TRIM9 (Q9C026, NP_443210). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Platypus, Xenopus (100%); Stickleback (92%); Panda, Chicken, Lizard, Zebrafish (85%).
Usage
ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.