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TRIM9 antibody LS-C80105 is an unconjugated rabbit polyclonal antibody to TRIM9 (SPRING) from human, mouse, rat and other species. Validated for WB.
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Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
Immunofluorescence, Western blot, Flow Cytometry
Product Description
TRIM9 antibody LS-C80105 is an unconjugated rabbit polyclonal antibody to TRIM9 (SPRING) from human, mouse, rat and other species. Validated for WB.
About SPRING / TRIM9
E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions and may also participate in the formation or breakdown of abnormal inclusions in neurodegenerative disorders. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.
Q9C026
NM_015163
NP_055978.4
TRIM9 Antibody, KIAA0282 Antibody, RING finger protein 91 Antibody, SPRING Antibody, Tripartite motif containing 9 Antibody, RNF91 Antibody, Tripartite motif-containing 9 Antibody
Specifications
Target
Human SPRING / TRIM9
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot
- (applications tested for the base form of this product only)
Immunogen
SPRING / TRIM9 antibody was raised against synthetic peptide from C-Terminus of human TRIM9 (Q9C026, NP_443210). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Platypus, Xenopus (100%); Stickleback (92%); Panda, Chicken, Lizard, Zebrafish (85%).
Blocking Peptide
LS-E12367 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80105. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C80105. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human TRIM9
Usage
ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
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Images
Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: United States
Date Requested: 9/26/2018
Date Requested: 9/26/2018
