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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPRING / TRIM9 Antibody (C-Terminus) IHC-plus™ LS-B1111

Note: This antibody replaces LS-C29873
Anti-TRIM9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM9 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TRIM9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM9 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SPRING / TRIM9 Antibody (C-Terminus) IHC-plus™ LS-B1111

Note: This antibody replaces LS-C29873
Antibody:
Rabbit Polyclonal to Human SPRING / TRIM9
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B1111-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SPRING / TRIM9
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
TRIM9 antibody LS-B1111 is an unconjugated rabbit polyclonal antibody to human TRIM9 (SPRING). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human SPRING / TRIM9
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SPRING / TRIM9 antibody was raised against synthetic peptide from C-Terminus of human TRIM9 (Q9C026-5, NP_443210). Percent identity by BLAST analysis: Human, Chimpanzee (100%); Rabbit (92%).
Blocking Peptide
LS-E12416 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1111. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human TRIM9
Usage
Immunohistochemistry: LS-B1111 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1111 was determined to be 2.5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPRING / TRIM9
Q9C026 NM_015163 NP_055978.4

Validation

Anti-TRIM9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-TRIM9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TRIM9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Product
Species: Hamster, Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep, Chicken
Applications: IHC, ICC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Requested From: United States
Date Requested: 6/25/2019
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