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SPP1 / Osteopontin Antibody IHC-plus™ LS-B10122

Note: This antibody replaces LS-C201116
Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 ug/ml.
Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 ug/ml.
Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 ug/ml.
Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 ug/ml.
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SPP1 / Osteopontin Antibody IHC-plus™ LS-B10122

Note: This antibody replaces LS-C201116
Antibody:
Rabbit Polyclonal to Human SPP1 / Osteopontin
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10122-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SPP1 / Osteopontin
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Osteopontin antibody LS-B10122 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human SPP1 / Osteopontin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (20 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
An 18 amino acid peptide near the amino terminus of human SPP1. The immunogen is located within amino acids 50 - 100 of SPP1.
Blocking Peptide
LS-E31467 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B10122. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.
Usage
SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 36 kDa Observed: 37 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPP1 / Osteopontin
P10451 NM_000582 NP_000573.1

Validation

Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SPP1 / Osteopontin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Requested From: United States
Date Requested: 6/24/2019
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