SPO11 antibody LS-C81959 is an unconjugated rabbit polyclonal antibody to SPO11 from human, bat, bovine and other species. Validated for WB.
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Pig, Zebrafish
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
SPO11 antibody was raised against synthetic peptide located between aa110-159 of human SPO11 (Q9Y5K1, NP_036576). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Bat, Pig, Opossum, Guinea pig (100%); Mouse, Rat, Rabbit, Horse, Zebra finch, Platypus (92%); Turkey, Chicken (84%); Stickleback (81%).
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.