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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPO11 Antibody (aa110‑159, Biotin) LS‑C459318
SPO11 antibody LS-C459318 is a biotin-conjugated rabbit polyclonal antibody to SPO11 from human, bat, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C459318-100
100 µl (1 mg/ml)
$385
Description
SPO11 antibody LS-C459318 is a biotin-conjugated rabbit polyclonal antibody to SPO11 from human, bat, bovine and other species. Validated for WB.
Target
Human SPO11
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Pig, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
SPO11 antibody was raised against synthetic peptide located between aa110-159 of human SPO11 (Q9Y5K1, NP_036576). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Bat, Pig, Opossum, Guinea pig (100%); Mouse, Rat, Rabbit, Horse, Zebra finch, Platypus (92%); Turkey, Chicken (84%); Stickleback (81%).
Epitope
aa110-159
Specificity
Human SPO11
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About SPO11
Q9Y5K1 NM_012444 NP_036576.1

Popular SPO11 Products

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bat, Bovine, Dog, Guinea pig, Pig, Zebrafish
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Western Blot Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate All Lanes: SPO11 antibody at 5.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 41 KDa Observed band size: 45 KDa
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

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Images

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/10/2018
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