Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
HPLC-purified human sperm phospholipase A2 (PLA2).
Specificity
Recognizes human type II secretory PLA2 at 14kD. Recognizes PLA2 in sperm, disc and synovial fluid.
Applications
- Western blot (5 - 10 µg/ml)
- ELISA (1:300)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 5-10 ug/ml detects 10 ng PLA2 under non-reducing conditions. Human synovial PLA2 was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PLA2, secretory (5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. ELISA: 1:300 detects 3-28 ng PLA2.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee