Target
Human SP140
Synonyms
SP140 | Nuclear autoantigen Sp-140 | Speckled 140 kDa | LYSP100 | SP140 nuclear body protein | LYSP100-A | LYSP100-B | Nuclear body protein SP140
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence EGQNLQEQVCPEP, from the N-Terminus of protein sequence according to NP_009168.4NP_001005176.1NP_001265380.1NP_001265381.1NP_001265382.1.
Specificity
This antibody is expected to recognize all reported isoforms (NP_009168.4; NP_001005176.1; NP_001265380.1; NP_001265381.1; NP_001265382.1).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 140kDa band observed in Human Spleen lysates (calculated MW of 98.2kDa according to NP_009168.4). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee