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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SOX2 Antibody (N‑Terminus, clone SP76) LS‑C210330
SOX2 antibody LS-C210330 is an unconjugated rabbit monoclonal antibody to human SOX2. Validated for IHC and WB.
Catalog
Size
Price
LS-C210330-500
500 µl
$485
Description
SOX2 antibody LS-C210330 is an unconjugated rabbit monoclonal antibody to human SOX2. Validated for IHC and WB.
Target
Human SOX2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Human, Bovine, Dog, Frog (at least 90% immunogen sequence identity)
Clonality
IgG Monoclonal [SP76]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:25)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SOX2 antibody was raised against synthetic peptide corresponding to N-terminus of human SOX-2 protein.
Epitope
N-Terminus
Specificity
Human SOX2
Usage
IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. IHC Positive control: Newborn brain. WESTERN BLOT: Recommended starting protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate. actual results may differ because of variability in methods and protocols. Positive Control: MCF7 cell lysate.
Presentation
10 mM PBS, pH 7.4, 0.1% Sodium Azide, BSA
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SOX2
P48431 L07335 AAA35997.1

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Images

Immunohistochemistry

Immunohistochemistry of Human Bladder stained with anti-Sox-2 antibody
Immunohistochemistry of Human Bladder stained with anti-Sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human Newborn Brain stained with anti-SOX-2 antibody
Immunohistochemistry of Human Newborn Brain stained with anti-SOX-2 antibody

Immunohistochemistry

Immunohistochemistry of Human lung stained with anti-sox-2 antibody
Immunohistochemistry of Human lung stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human lung squamous cell carcinoma stained with anti-sox-2 antibody
Immunohistochemistry of Human lung squamous cell carcinoma stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human ovarian adenocarcinoma stained with anti-sox-2 antibody
Immunohistochemistry of Human ovarian adenocarcinoma stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human Bladder stained with anti-Sox-2 antibody
Immunohistochemistry of Human Bladder stained with anti-Sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human Newborn Brain stained with anti-SOX-2 antibody
Immunohistochemistry of Human Newborn Brain stained with anti-SOX-2 antibody

Immunohistochemistry

Immunohistochemistry of Human lung stained with anti-sox-2 antibody
Immunohistochemistry of Human lung stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human lung squamous cell carcinoma stained with anti-sox-2 antibody
Immunohistochemistry of Human lung squamous cell carcinoma stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human ovarian adenocarcinoma stained with anti-sox-2 antibody
Immunohistochemistry of Human ovarian adenocarcinoma stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human Bladder stained with anti-Sox-2 antibody
Immunohistochemistry of Human Bladder stained with anti-Sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human Newborn Brain stained with anti-SOX-2 antibody
Immunohistochemistry of Human Newborn Brain stained with anti-SOX-2 antibody

Immunohistochemistry

Immunohistochemistry of Human lung stained with anti-sox-2 antibody
Immunohistochemistry of Human lung stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human lung squamous cell carcinoma stained with anti-sox-2 antibody
Immunohistochemistry of Human lung squamous cell carcinoma stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human ovarian adenocarcinoma stained with anti-sox-2 antibody
Immunohistochemistry of Human ovarian adenocarcinoma stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human Bladder stained with anti-Sox-2 antibody
Immunohistochemistry of Human Bladder stained with anti-Sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human Newborn Brain stained with anti-SOX-2 antibody
Immunohistochemistry of Human Newborn Brain stained with anti-SOX-2 antibody

Immunohistochemistry

Immunohistochemistry of Human lung stained with anti-sox-2 antibody
Immunohistochemistry of Human lung stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human lung squamous cell carcinoma stained with anti-sox-2 antibody
Immunohistochemistry of Human lung squamous cell carcinoma stained with anti-sox-2 antibody

Immunohistochemistry

Immunohistochemistry of Human ovarian adenocarcinoma stained with anti-sox-2 antibody
Immunohistochemistry of Human ovarian adenocarcinoma stained with anti-sox-2 antibody

Requested From: United States
Date Requested: 12/10/2018
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