SOX2 antibody LS-C210330 is an unconjugated rabbit monoclonal antibody to human SOX2. Validated for IHC and WB.
(tested or 100% immunogen sequence identity)
Human, Bovine, Dog, Frog (at least 90% immunogen sequence identity)
Protein A/G purified
- IHC - Paraffin (1:100)
- Western blot (1:25)
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SOX2 antibody was raised against synthetic peptide corresponding to N-terminus of human SOX-2 protein.
IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. IHC Positive control: Newborn brain. WESTERN BLOT: Recommended starting protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate. actual results may differ because of variability in methods and protocols. Positive Control: MCF7 cell lysate.
10 mM PBS, pH 7.4, 0.1% Sodium Azide, BSA
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.