SOX2 antibody LS-C210330 is an unconjugated rabbit monoclonal antibody to human SOX2. Validated for IHC and WB.
(tested or 100% immunogen sequence identity)
Human, Bovine, Dog, Frog (at least 90% immunogen sequence identity)
Protein A/G purified
- IHC - Paraffin (1:100)
- Western blot (1:25)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
SOX2 antibody was raised against synthetic peptide corresponding to N-terminus of human SOX-2 protein.
IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. IHC Positive control: Newborn brain. WESTERN BLOT: Recommended starting protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate. actual results may differ because of variability in methods and protocols. Positive Control: MCF7 cell lysate.
10 mM PBS, pH 7.4, 0.1% Sodium Azide, BSA
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.