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Antibodies > SOD2 / Mn SOD > LS-C312936
 
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Catalog Number Size Price
LS-C312936-10 10 µg $318 
LS-C312936-100 100 µg (0.5 mg/ml) $470 
SOD2 / Mn SOD Antibody - IHC analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody. SOD2/Mnsod was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SOD2/Mnsod Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SOD2 / Mn SOD Antibody - MNSOD / SOD2 antibody. IHC(P): Human Intestinal Cancer Tissue.
SOD2 / Mn SOD Antibody - MNSOD / SOD2 antibody. IHC(P): Rat Brain Tissue.
SOD2 / Mn SOD Antibody - Western blot analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse small intestine tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2/Mnsod antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOD2/Mnsod at approximately 25KD. The expected band size for SOD2/Mnsod is at 25KD.
SOD2 / Mn SOD Antibody - Western blot analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Intestine Tissue Lysate, Lane 3: Rat Lung Tissue Lysate, Lane 4: Rat Heart Tissue Lysate, Lane 5: SMMC Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: COLO320 Whole Cell Lysate, Lane 8: SW620 Whole Cell Lysate, Lane 9: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2/Mnsod antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOD2/Mnsod at approximately 25KD. The expected band size for SOD2/Mnsod is at 25KD.
SOD2 / Mn SOD Antibody - WB of MNSOD / SOD2 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Intestines Tissue Lysate. Lane 3: Rat Lung Tissue Lysate. Lane 4: Rat Heart Tissue Lysate. Lane 5: SMMC Cell Lysate . Lane 6: HELA Cell Lysate. Lane 7: COLO320 Cell Lysate. Lane 8: SW620 Cell Lysate . Lane 9: A549 Cell Lysate.
SOD2 / Mn SOD Antibody - IHC analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody. SOD2/Mnsod was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SOD2/Mnsod Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SOD2 / Mn SOD Antibody - MNSOD / SOD2 antibody. IHC(P): Human Intestinal Cancer Tissue.
SOD2 / Mn SOD Antibody - MNSOD / SOD2 antibody. IHC(P): Rat Brain Tissue.
SOD2 / Mn SOD Antibody - Western blot analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse small intestine tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2/Mnsod antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOD2/Mnsod at approximately 25KD. The expected band size for SOD2/Mnsod is at 25KD.
SOD2 / Mn SOD Antibody - Western blot analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Intestine Tissue Lysate, Lane 3: Rat Lung Tissue Lysate, Lane 4: Rat Heart Tissue Lysate, Lane 5: SMMC Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: COLO320 Whole Cell Lysate, Lane 8: SW620 Whole Cell Lysate, Lane 9: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2/Mnsod antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOD2/Mnsod at approximately 25KD. The expected band size for SOD2/Mnsod is at 25KD.
SOD2 / Mn SOD Antibody - WB of MNSOD / SOD2 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Intestines Tissue Lysate. Lane 3: Rat Lung Tissue Lysate. Lane 4: Rat Heart Tissue Lysate. Lane 5: SMMC Cell Lysate . Lane 6: HELA Cell Lysate. Lane 7: COLO320 Cell Lysate. Lane 8: SW620 Cell Lysate . Lane 9: A549 Cell Lysate.
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Polyclonal Rabbit anti‑Human SOD2 / Mn SOD Antibody (aa45‑62, IHC, WB) LS‑C312936

Polyclonal Rabbit anti‑Human SOD2 / Mn SOD Antibody (aa45‑62, IHC, WB) LS‑C312936

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Note: This antibody replaces LS-C389243
Antibody:
SOD2 / Mn SOD Rabbit anti-Human Polyclonal (aa45-62) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312936-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SOD2 / Mn SOD Rabbit anti-Human Polyclonal (aa45-62) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Mn SOD antibody LS-C312936 is an unconjugated rabbit polyclonal antibody to Mn SOD (SOD2) (aa45-62) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human SOD2 / Mn SOD
Synonyms
SOD2 | IPOB | MVCD6 | Indophenoloxidase B | Mn superoxide dismutase | MNSOD | Mangano-superoxide dismutase
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human SOD2(45-62 aa QIMQLHHSKHHAAYVNNL), identical to the related mouse sequence and different from the related rat sequence by one amino acid.
Epitope
aa45-62
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SOD2 / Mn SOD
P04179 NM_000636 NP_000627.2

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IHC‑plus™ SOD2 / Mn SOD Antibody LS‑B3694
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SOD2 / Mn SOD Antibody - Anti-SOD2 / MNSOD antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
PathPlus™ SOD2 / Mn SOD Antibody LS‑B3161
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SOD2 / Mn SOD Antibody - Anti-MNSOD / SOD2 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
PathPlus™ SOD2 / Mn SOD Antibody LS‑B9291
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SOD2 / Mn SOD Antibody - Anti-MNSOD / SOD2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PathPlus™ SOD2 / Mn SOD Antibody LS‑B8169
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus, Gerbil
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
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Species: Human, Mouse, Rat
Applications: IHC, IHC - Frozen, ICC, Western blot
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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