Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > SOCS3 > LS-C312859
 
Print Page  
Catalog Number Size Price
LS-C312859-10 10 µg $318 
LS-C312859-100 100 µg (0.5 mg/ml) $470 
SOCS3 Antibody - IHC analysis of SOCS3 using anti-SOCS3 antibody. SOCS3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SOCS3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SOCS3 Antibody - Western blot analysis of SOCS3 using anti-SOCS3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOCS3 at approximately 30KD. The expected band size for SOCS3 is at 25KD.
SOCS3 Antibody - WB of SOCS3 antibody. Lane 1: JURKAT Cell Lysate. Lane 2: CEM Cell Lysate. Lane 3: RAJI Cell Lysate.
SOCS3 Antibody - IHC analysis of SOCS3 using anti-SOCS3 antibody. SOCS3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SOCS3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SOCS3 Antibody - Western blot analysis of SOCS3 using anti-SOCS3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOCS3 at approximately 30KD. The expected band size for SOCS3 is at 25KD.
SOCS3 Antibody - WB of SOCS3 antibody. Lane 1: JURKAT Cell Lysate. Lane 2: CEM Cell Lysate. Lane 3: RAJI Cell Lysate.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human SOCS3 Antibody (aa71‑85, IHC, WB) LS‑C312859

Polyclonal Rabbit anti‑Human SOCS3 Antibody (aa71‑85, IHC, WB) LS‑C312859

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C389264
Antibody:
SOCS3 Rabbit anti-Human Polyclonal (aa71-85) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Sheep, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312859-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SOCS3 Rabbit anti-Human Polyclonal (aa71-85) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Sheep, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
SOCS3 antibody LS-C312859 is an unconjugated rabbit polyclonal antibody to SOCS3 (aa71-85) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human SOCS3
Synonyms
SOCS3 | Cish3 | Cytokine-induced SH2 protein 3 | CIS-3 | CIS3 | SSI-3 | STAT-induced STAT inhibitor 3 | SSI3 | ATOD4 | SOCS-3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Sheep, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SOCS3(71-85 aa RDSSDQRHFFTLSVK), identical to the related mouse sequence, different from the related rat sequence by one amino acid.
Epitope
aa71-85
Specificity
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SOCS3
O14543 NM_003955 NP_003946.3

Publications (0)

Submit a Publication for LS-C312859

Customer Reviews (0)

Submit a review for LS-C312859

Featured Products

SOCS3 Antibody - Anti-SOCS3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3.
SOCS3 Antibody (clone OTI11C5) LS‑C172981
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
SOCS3 Antibody - Western blot analysis of SOCS3 expression in MOLT4 (A); A431 (B); MCF7 (C); Raw264.7 (D); H9C2 (E) whole cell lysates.
SOCS3 Antibody (Internal) LS‑C353123
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Chicken
Applications: Western blot
SOCS3 Antibody - Western Blot; Sample: Mouse Cerebrum lysate; Primary Ab: 2µg/ml Rabbit Anti-Mouse SOCS3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
SOCS3 Antibody (aa1‑225, Biotin) LS‑C297684
Species: Mouse
Applications: Western blot
SOCS3 Antibody - IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SOCS3 mouse monoclonal antibody.
SOCS3 Antibody (clone OTI8G3) LS‑C173025
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
SOCS3 Antibody - SOCS3 antibody (1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
SOCS3 Antibody (aa213‑225) LS‑C55482
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
SOCS3 Antibody - IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-SOCS3 mouse monoclonal antibody.
SOCS3 Antibody (clone OTI5D4) LS‑C172776
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
See all SOCS3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com