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Catalog Number Size Price
LS-C773096-100 100 µg (1 mg/ml) $475 
SNCA / Alpha-Synuclein Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.
SNCA / Alpha-Synuclein Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 µg/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody  at 1:200 for 24 hours at 4°C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) Alpha Synuclein Antibody. (D) Composite.
SNCA / Alpha-Synuclein Antibody - Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa.
SNCA / Alpha-Synuclein Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.
SNCA / Alpha-Synuclein Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 µg/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody  at 1:200 for 24 hours at 4°C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) Alpha Synuclein Antibody. (D) Composite.
SNCA / Alpha-Synuclein Antibody - Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa.
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Monoclonal Mouse anti‑Mouse SNCA / Alpha‑Synuclein Antibody (clone 3F8, Biotin, IHC, IF, WB) LS‑C773096

Monoclonal Mouse anti‑Mouse SNCA / Alpha‑Synuclein Antibody (clone 3F8, Biotin, IHC, IF, WB) LS‑C773096

Antibody:
SNCA / Alpha-Synuclein Mouse anti-Mouse Monoclonal (Biotin) (3F8) Antibody
Application:
IHC, IF, WB, ELISA, DB
Reactivity:
Mouse, Human, Rat
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$475
LS-C773096-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SNCA / Alpha-Synuclein Mouse anti-Mouse Monoclonal (Biotin) (3F8) Antibody
Application:
IHC, IF, WB, ELISA, DB
Reactivity:
Mouse, Human, Rat
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
Alpha-Synuclein antibody LS-C773096 is a biotin-conjugated mouse monoclonal antibody to Alpha-Synuclein (SNCA) from mouse. It is reactive with human, mouse and rat. Validated for DB, ELISA, IF, IHC and WB.
Target
Mouse SNCA / Alpha-Synuclein
Synonyms
SNCA | Alpha-synuclein | PARK4 | PD1 | Synuclein alpha-140 | NACP | PARK1 | aSynuclein | a-Synuclein
Host
Mouse
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
3F8
Conjugations
Biotin. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 594, FITC, PerCP, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Mouse alpha synuclein aggregate
Specificity
Binds within the 101-140 aa region of human alpha synuclein, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein.
Applications
  • IHC (1:200)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • ELISA (1:1000)
  • Dot Blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Protect from light. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SNCA / Alpha-Synuclein
P37840 NM_000345 NP_000336.1

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Requested From: United States
Date Requested: 4/17/2024