SLUG antibody LS-C407652 is an unconjugated rabbit polyclonal antibody to SLUG (SNAI2) from human, mouse, rat and other species. Validated for IHC and WB.
Human SNAI2 / SLUG
Human, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Hamster (at least 90% immunogen sequence identity)
Immunogen affinity purified
- IHC - Paraffin (0.5 - 1 µg/ml)
- Western blot (0.1 - 0.5 µg/ml)
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SNAI2 / SLUG antibody was raised against a synthetic peptide corresponding to a sequence in the middle region of human SLUG (116-148aa KLSDPHAIEAEKFQCNLCNKTYSTFSGLAKHKQ), identical to the related mouse and rat sequences.
Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level). .
WB: The detection limit for SLUG is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Lyophilized from 7.1 mM sodium phosphate, 77 mM NaCl, 2.5% BSA, 0.025% sodium azide
Reconstitute with 200 µl of distilled water.
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
For research use only.