Target
Human SMC1A / SMC1
Synonyms
SMC1A | CDLS2 | DXS423E | KIAA0178 | SB1.8 | SMC protein 1A | SMC1 | SMC1L1 | SMC-1-alpha | SMC-1A | SMCB
Reactivity
Human, Monkey, Bovine, Dog, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
KLH-conjugated, synthetic peptide corresponding to aa 951-962, (SQEEGS[pS]QGEDS) of human SMC1 (Structural Maintenance of Chromosome 1). The immunizing sequence is identical in bovine and has 11/12 identical amino acids in mouse and xenopus.
Specificity
Recognizes phospho-SMC1 (Ser957), Mr 150kD. Species cross-reactivity: Human and mouse. Predicted to cross-react with Xenopus and bovine based on sequence homology.
Applications
- Western blot (1 - 2 µg/ml)
Usage
Suitable for use in Western Blot. Western Blot: 1-2 ug/ml detects phospho-SMC1 in RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells. Cell lysates from untreated and irradiated GMO536 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SMC1 (Ser957) (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee