Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C161303-400 400 µl $393 
SMAD9 Antibody - Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
SMAD9 Antibody - SMAD9 Antibody western blot of A549 cell line lysates (35 ug/lane). The SMAD9 antibody detected the SMAD9 protein (arrow).
SMAD9 Antibody - Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
SMAD9 Antibody - SMAD9 Antibody western blot of A549 cell line lysates (35 ug/lane). The SMAD9 antibody detected the SMAD9 protein (arrow).
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human SMAD9 Antibody (aa200‑228, IF, WB) LS‑C161303

Polyclonal Rabbit anti‑Human SMAD9 Antibody (aa200‑228, IF, WB) LS‑C161303

Antibody:
SMAD9 Rabbit anti-Human Polyclonal (aa200-228) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C161303-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SMAD9 Rabbit anti-Human Polyclonal (aa200-228) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD9 antibody LS-C161303 is an unconjugated rabbit polyclonal antibody to human SMAD9 (aa200-228). Validated for IF and WB. Cited in 1 publication.
Target
Human SMAD9
Synonyms
SMAD9 | MAD homolog 9 | SMAD 9 | SMAD8A | SMAD8 | Smad9 | MADH9 | SMAD8B | MADH6 | Mothers against DPP homolog 9 | SMAD family member 9
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa200-228
Specificity
This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9.
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD9

LSBio Ratings

SMAD9 Antibody (aa200-228) for IF/Immunofluorescence, WB/Western LS-C161303 has an LSBio Rating of
Publications (4)

Publications (1)

Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis. Donglim Esther Park, Jingwei Cheng, John P McGrath, Matthew Y Lim, Camille Cushman, Selene K Swanson, Michelle L Tillgren, Joao A Paulo, Prafulla C Gokhale, Laurence Florens, Michael P Washburn, Patrick Trojer, James A DeCaprio. Nature cell biology. 2020 May;22:603-615.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/20/2024