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Catalog Number Size Price
LS-C153867-100 100 µg (1 mg/ml) $567 
SMAD3 Antibody - Western blot of Rabbit Anti-SMAD3 antibody. Lane 1: AML12 unstimulated. Lane 2: AML12 stimulated with TGFB. Lane 3: MEFwt unstimulated. Lane 4: MEFwt stimulated with TGFB. Lane 5: MEF Smad3 KO unstimulated. Lane 6: MEF Smad3 KO stimulated with TGFB. Lane 7: HEK293 Smad3T179A mutant unstimulated. Lane 8: HEK293 Smad3T179A mutant stimulated with TGFB. Lane 9: HEK293 Smad3T179V mutant unstimulated. Lane 10: HEK293 Smad3T179V mutant stimulated with TGFB. Load: 35 ug per lane. Primary antibody: SMAD 3 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 48.1kDa. Other band(s): Smad2pT220.

Polyclonal Rabbit anti‑Human SMAD3 Antibody (aa179, Internal, WB) LS‑C153867

Polyclonal Rabbit anti‑Human SMAD3 Antibody (aa179, Internal, WB) LS‑C153867

Antibody:
SMAD3 Rabbit anti-Human Polyclonal (aa179, Internal) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C153867-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SMAD3 Rabbit anti-Human Polyclonal (aa179, Internal) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-C153867 is an unconjugated rabbit polyclonal antibody to human SMAD3 (aa179, Internal). Validated for ELISA and WB.
Target
Human SMAD3
Synonyms
SMAD3 | HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | HSMAD3 | Mad protein homolog | SMAD 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Marmoset, Mouse, Rat, Dog, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Epitope
aa179, Internal
Specificity
A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • Western blot (1:1000)
  • ELISA (1:15000 - 1:25000)
Usage
SMAD3 Antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kD in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/25/2024