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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C153867-100 100 µg (1 mg/ml) $425 
SMAD3 Antibody - Western blot of Rabbit Anti-SMAD3 antibody. Lane 1: AML12 unstimulated. Lane 2: AML12 stimulated with TGFB. Lane 3: MEFwt unstimulated. Lane 4: MEFwt stimulated with TGFB. Lane 5: MEF Smad3 KO unstimulated. Lane 6: MEF Smad3 KO stimulated with TGFB. Lane 7: HEK293 Smad3T179A mutant unstimulated. Lane 8: HEK293 Smad3T179A mutant stimulated with TGFB. Lane 9: HEK293 Smad3T179V mutant unstimulated. Lane 10: HEK293 Smad3T179V mutant stimulated with TGFB. Load: 35 ug per lane. Primary antibody: SMAD 3 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 48.1kDa. Other band(s): Smad2pT220.

Polyclonal SMAD3 Antibody (aa179, Internal, WB) LS‑C153867

Polyclonal SMAD3 Antibody (aa179, Internal, WB) LS‑C153867

Antibody:
Rabbit Polyclonal to Human SMAD3
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153867-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human SMAD3
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-C153867 is an unconjugated rabbit polyclonal antibody to human SMAD3 (aa179, Internal). Validated for ELISA and WB.
Target
Human SMAD3
Synonyms
SMAD3 | HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | HSMAD3 | Mad protein homolog | SMAD 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Marmoset, Mouse, Rat, Dog, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA (1:15000 - 1:25000)
Immunogen
SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Epitope
aa179, Internal
Specificity
A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Usage
SMAD3 Antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kD in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

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Requested From: United States
Date Requested: 4/6/2020